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New publication in ChemBioChem
"Cell-free protein synthesis for the screening of novel azoreductases and their preferred electron donor" was published in ChemBioChem.
Jascha Rolf, Anna Christina Reyes Ngo, Prof. Dr. Stephan Lütz, Prof. Dr. Dirk Tischler, Dr. Katrin Rosenthal
Abstract:
A screening approach for azoreductases was developed, including cell-free protein synthesis of an enzyme library and a multi-well microplate assay. Applicability to known and novel enzymes was demonstrated, and preferred co-substrates for each enzyme were evaluated. Five new and yet uncharacterized azoreductases were identified. Furthermore, it was shown that all azoreductases accept the co-substrate BNAH as an electron donor.