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New publication in Metabolic Engineering Communications
The publication "Metabolic bottlenecks of Pseudomonas taiwanensis VLB120 during growth on D-xylose via the Weimberg pathway" was published.
The microbial production of chemicals from renewable raw materials is an important step towards a sustainable, bio-based economy. Production strains must therefore be able to efficiently utilize the components obtained from lignocellulose, such as D-xylose. The versatile and robust strain Pseudomonas taiwanensis VLB120 assimilates D-xylose via the Weimberg pathway. In this work, we identified bottlenecks of P. taiwanensis VLB120 during growth on D-xylose. Xylonolactonase activity, D-xylonate import and extracellular pH were found to be crucial factors for the activity of the metabolic pathway. In addition, we identified two previously unknown D-xylonate transporters. Our study contributes to the fundamental knowledge of the Weimberg pathway in pseudomonads and shows how the metabolic bottlenecks of the Weimberg pathway can be addressed to advance the development of strains for bioprocesses with renewable feedstocks.