Regine Siedentop M.Sc.
CV
Career
Since 04/2019 PhD at the Chair of Bioprocess Engineering under Prof. Dr. Stephan Lütz, BCI, TU Dortmund, with the topic: "Iterative Optimization of an In Vitro Enzyme Cascade using Algorithm-based Analysis and Design".
06/2016 - 03/2019 Master of Science in Chemical Biology, TU Dortmund University, with the master thesis at the Bioprocess Engineering Department under Prof. Dr. Stephan Lütz: "Heterologous Expression and Activity Screening of Cyclic GMP-AMP Synthase Homologues for the Enzymatic Production of Cyclic Dinucleotides".
10/2012 - 05/2016 Bachelor of Science in Chemical Biology, TU Dortmund University, with the bachelor thesis at the Chemical Genomics Centre, MPI, Dortmund under Dr. Sven Hennig: "Method optimization for the analysis of RNA protein interactions using spinach derivatives".
Publications
Siedentop R, Siska, M, Möller N, Lanzrath H, von Lieres E, Lütz S, Rosenthal K, Bayesian Optimization for an ATP-Regenerating In Vitro Enzyme Cascade. Catalysts 2023, DOI
Siedentop R, Dziennus M, Lütz S, Rosenthal K Debottlenecking of an In Vitro Enzyme Cascade Using a Combined Model‐ and Experiment‐Based Approach. Chemie Ing. Tech. 2023 DOI
Kinner A., Nerke P., Siedentop R., Steinmetz T., Claassen T., Rosenthal K., Nett M., Pietruszka J., Lütz S. Recent Advances in Biocatalysis for Drug Synthesis Biomedicines, 2022, 10(5), 964 DOI.
Siedentop R., Rosenthal K. Industrially Relevant Enzyme Cascades for Drug Synthesis and Their Ecological Assessment International Journal of Molecular Science, 2022, 23(7), 3605 DOI
Siedentop R., Claaßen C., Rother D., Lütz S., Rosenthal K. Getting the Most Out of Enzyme Cascades: Strategies to Optimize In Vitro Multi-Enzymatic Reactions Catalysts, 2021, 11, 1183 DOI
Rosenthal K., Becker M., Rolf J., Siedentop R., Hillen M., Nett M., and Lütz S. (2020) Catalytic promiscuity of cGAS: A facile enzymatic synthesis of 2'-3' linked cyclic dinucleotides ChemBioChem, DOI.
Rolf J., Siedentop R., Lütz S., and Rosenthal K. (2020) Screening and Identification of Novel cGAS Homologues Using a Combination of In Vitro and In Vivo Protein Synthesis International Journal of Molecular Science, 21(1): 105
Poster
Siedentop R., Dziennus M., Lütz S., Rosenthal K. (selected for Poster Flash Talk) Identification of Optimization Parameters in Cell-free Enzyme Cascades by Computer Modeling and Experimental Validation (Best poster award) 5th Multistep Enzyme Catalyzed Processes Conference (MECP2020+1), September 13-16, 2021, online.
Siedentop R., Dziennus M., Lütz S., Rosenthal K. Optimization of an Enzymatic Cascade using a Mechanistic Model Himmelfahrtstagung on Bioprocess Engineering 2022. May 23-25, 2022, Mainz, Germany.
Rosenthal K., Becker M., Rolf J., Siedentop R., and Lütz S. (selected for Poster Pitch Talk) Single-step reaction and enzyme cascade - Biocatalytic synthesis routes for novel cyclic dinucleotides 15th International Symposium on Biocatalysis and Biotransformations, July 19-22, 2021, online.
Rosenthal K., Rolf J., Becker M., Siedentop R., and Lütz S. Cell-free synthesis of enzymes for the production of pharmaceutically relevant molecules ProcessNet Annual Meeting, September 21-24, 2020, online.
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